Fig 6. CJB111 EsxA is a pore-forming protein.

Lipid bilayers composed of 1,2-diphytanoyl-sn-glycerol-3-phosphocholine (DphpC), 4ME 16:0 PC were incubated with 5 μg CJB111 recombinant EsxA at a) pH 7 and b) pH 4.0 in 25 mM sodium phosphate 1M KCl. Representative current traces are shown, and insertion sizes and frequencies are summarized in the histograms. Ten membranes were run in each buffer condition. No protein controls were also performed to rule out artifactual pore formation due to contamination of the system or buffer. Additional current traces can be found in S5A and S5B Fig. c) Transmission electron microscopy of recombinant EsxA1. Shown are EsxA1 particles negatively stained with 1% uranyl formate and selected reference-free 2D class averages from 12,727 particles that resembled an intact oligomeric pore. The full set of class averages can be found in S5C Fig.