Figure 8.

RAW 264.7 macrophages were treated with 10 μM CAL-101, a specific inhibitor of p110δ, the catalytic subunit of PI3Kδ isoform, 30 min before the addition of 15 μM benznidazole (Bzl). Cells were stimulated with 10 µg/mL LPS 30 min after Bzl treatment. After 48 h, NO levels were quantified by the Griess reaction in culture supernatants (A) and relative Arginase I (~35-38 kDa) protein expression was determined by Western blot with a specific antibody and normalized against β-actin (43 kDa) (B). Results are expressed as the mean of 3 independent experiments (n=3, 5 replicates/treatment) ± SEM. ***P < 0.0001 vs. control cells, ^### P < 0.0001 vs LPS-stimulated cells. ^ϕ P < 0.05 vs. Bzl-treated and LPS-stimulated cells.