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. 2021 Dec 2;12:782891. doi: 10.3389/fimmu.2021.782891

Table 1.

Primer sequences used for RT-qPCR, including melting temperature and amplicon size, were provided.

Forward (5’-3’) Reverse (5’-3’) Tm (°C) Amplicon size (bp)
β-actin: GGCTGTATTCCCCTCCATCG CCAGTTGGTAACAATGCCATGT 62 241
IL-6: TGATGCACTTGCAGAAAACAA GGTCTTGGTCCTTAGCCACTC 60 270
IL-17A: GAAGGCAGCAGCGATCATC CGTTTCCCTCCGCATTGACA 65 255
MR: CAAGGAAGGTTGGCATTTGT CCTTTCAGTCCTTTGCAAGC 60 111
NOS2: CACAGCAATATAGGCTCATCCA GGATTTCAGCCTCATGGTAAAC 60 101
PPAR-α: CCATACAGGAGAGCAGGGATTT TTACCTACGCTCAGCCCTCTTC 62 76
PPAR-γ: ATCTACACGATGCTGGC GGATGTCCTCGATGGG 60 253
SOCS3: CCTTTGACAAGCGGACTCTC CCTTTGACAAGCGGACTCTC 60 216
TNF-α: CGGGCAGGTCTACTTTGGAG ACCCTGAGCCATAATCCCCT 62 166
VEGF-A: TGCGGGATCAAACCTCACCA TCTCCGCTCTGAACAAGG 62 453

bp, base pairs.