Table 6.

Effect of different cryoprotectants on the particle size, PdI, zeta potential and dispersion time of freeze dried LNCs

Cryoprotectants	Concentration %, w/w	Particle size, nm	PdI	Zeta potential, mV	Dispersion time, S
PEG4000	1	113.60 ± 1.47	0.22 ± 0.01	−43.23 ± 0.68	105
	3	135.73 ± 3.15	0.31 ± 0.01	−49.46 ± 0.60	75
	5	162.36 ± 3.45	0.37 ± 0.01	−51.10 ± 0.79	40
sorbitol	1	116.00 ± 5.88	0.32 ± 0.04	−48.96 ± 3.76	120
	3	126.90 ± 0.80	0.24 ± 0.02	−44.53 ± 2.83	90
	5	250.86 ± 10.51	0.40 ± 0.02	−50.93 ± 3.08	90
sucrose	1	108.63 ± 1.15	0.23 ± 0.01	−50.23 ± 1.85	30
	3	115.36 ± 1.42	0.21 ± 0.01	−43.26 ± 0.23	20
	5	128.36 ± 4.56	0.35 ± 0.00	−44.56 ± 0.23	10
aerosil	1	177.33 ± 2.17	0.40 ± 0.02	−49.76 ± 3.00	45
	3	233.76 ± 25.83	0.38 ± 0.06	−49.93 ± 2.05	40
	5	436.80 ± 44.38	0.52 ± 0.17	−45.70 ± 1.41	40
lactose	1	121.06 ± 1.68	0.28 ± 0.03	−53.90 ± 2.36	60
	3	158.13 ± 33.96	0.36 ± 0.03	−54.13 ± 1.09	50
	5	149.23 ± 21.01	0.28 ± 0.05	−54.50 ± 1.41	30