FIG. 2.

Specific PCR amplification of the spirochetal rpoB gene. The PCR assay was performed by using Taq polymerase with an annealing temperature of 52°C. Lanes: 1, molecular mass markers (DNA molecular weight marker VI; Boehringer); 2, Borrelia burgdorferi; 3, Borrelia recurrentis; 4, Treponema pallidum; 5, Leptospira biflexa serovar patoc; 6, Leptospira interrogans, serovar australis; 7, Leptospira interrogans, serovar icterohaemmorragiae; 8, Escherichia coli; 9, Staphylococcus aureus; 10, Streptococcus salivarius; 11, Pseudomonas aeruginosa; 12, negative control without DNA. (A) rpoB gene primers LTB 1730F and LTB 2900R. (B) rpoB gene primers LTB 1730F and LTB 3700R. (C) 16S rRNA gene primers FD1 and RP2.