| Strain, strain background(Mus musculus, males and females) |
Esr1-/-C57BL/6 J(Symbol: Esr1tm1.1Mma, Synonyme: ERalpha Knockout) |
Mouse Clinical Inst., Strasbourg, France, Dupont et al., 2000
|
|
MGI:2386760 |
| Strain, strain background(Mus musculus, males) |
AF2°ERα,C57BL/6 J(Symbol: Esr1tm1.1Ohl Synonym: ERalpha-AF2°) |
Mouse Clinical Inst., Strasbourg, France, Billon-Galés et al., 2009
|
|
MGI:4950046 |
| Strain, strain background(Mus musculus, males) |
C451A-ERα, C57BL/6 N(Symbol: Esr1tm1.1Ics Synonyme: C451A-ERalpha knock-in) |
Mouse Clinical Inst., Strasbourg, France, Adlanmerini et al., 2014
|
|
MGI:5574591 |
| Strain, strain background(Mus musculus, males) |
TekCre/+:Esr1f/f, C57BL/6(B6.Cg-Tg(Tek-cre)12Flv/J backcrossed with Esr1tm1.2MmaSynonym:Tie2Cre ERαlox/lox) |
Esr1lox/lox: Mouse Clinical Institut, Strasbourg, France.TekCre: Jackson Lab (Bar Harbor, Me), Billon-Galés et al., 2009TekCre:Koni et al., 2001Esr1lox/lox:Dupont et al., 2000
|
TekCre:Esr1lox/lox: |
MGI:3775510 |
| Strain, strain background(Mus musculus, males) |
Esr2-/-,C57BL/6 J (Symbol: Esr2tm1MmaSynonym: ERbeta) |
Mouse Clinical Inst., Strasbourg, France, Dupont et al., 2000
|
|
MGI:2386761 |
| Strain, strain background(Mus musculus, males) |
R264A-ERα, C57BL/6 N |
Mouse Clinical Inst., Strasbourg, France, Adlanmerini et al., 2020
|
|
No MGI ID yet |
| Antibody |
Anti-eNOS, (mouse monoclonal, clone3) |
BD Biosciences |
Cat# 610297, RRID:AB_397691
|
WB (1:1000) |
| Antibody |
Anti-phospho-eNOS, pS1177 (Mouse monoclonal,Clone 19/eNOS/S1177) |
BD Biosciences |
Cat# 612392, RRID:AB_399750
|
WB (1:1000) |
| Antibody |
Anti-beta-actin, (Mouse monoclonal, clone AC-74) |
Sigma-Aldrich |
Cat#: 5316; RRID:AB_476743
|
WB (1:5000) |
| Antibody |
Anti-Akt Pan, (rabbit monoclonal, clone C67E7) |
Cell signalling technologyOzyme |
Cat#: 4691; RRID:AB_915783
|
WB (1:1000) |
| Antibody |
Anti-phospho-Akt, S473, (rabbit monoclonal, clone D9E) |
Cell signalling technology Ozyme |
Cat#: 4060; RRID:AB_2315049
|
WB (1:2000) |
| Antibody |
Anti-mouse IgG (H + L) Secondary antibody HRP (Goat polyclonal) |
Thermo scientific |
Cat#: 31430; RRID:AB_228307
|
WB (1:5000) |
| Antibody |
Anti-rabbit IgG(H + L) Secondary antibody HRP (Goat polyclonal) |
Thermo scientific |
Cat#: 31460; RRID:AB_228341
|
WB (1:10000) |
| Chemical compound, drug |
vitamin C |
Sigma Aldrich Merck, Favre et al., 2011
|
A5960 |
|
| Chemical compound, drug |
vitamin E |
Sigma Aldrich Merck, Favre et al., 2011
|
T3251 |
|
| Chemical compound, drug |
Mito-tempo |
Sigma Aldrich Merck, Freed et al., 2014
|
SML0737 |
|
| Chemical compound, drug |
catalase |
Sigma Aldrich Merck, Bouvet et al., 2007
|
C3155 |
|
| Chemical compound, drug |
PEG-superoxide dismutase (SOD) |
Sigma Aldrich Merck, Bouvet et al., 2007
|
S9549 |
|
| Chemical compound, drug |
Estetrol (E4) |
Sigma Aldrich Merck, Abot et al., 2014
|
SML1523 |
|
| Chemical compound, drug |
ICI 182 780 |
Tocris Biotechne, Meyer et al., 2010
|
1047 |
|
| Chemical compound, drug |
G-1 ((±)–1-[(3aR*,4S*,9bS*)–4-(6-Bromo-1,3-benzodioxol-5-yl)–3 a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinolin-8-yl)]- ethanone |
Cayman chemical Bertin Bioreagent, Meyer et al., 2010
|
10008933 |
|
| chemical compound, drug |
G-36 ((±)-(3aR*,4S*,9bS*)–4-(6-Bromo-1,3-benzodioxol-5-yl)–3 a,4,5,9b-tetrahydro-8-(1-methylethyl))–3H-cyclopenta[c]quinoline |
Cayman chemical Bertin Bioreagent, Meyer et al., 2016
|
14,397 |
|
| Sequence-based reagent |
N-(methylsulfonyl)–2-(2-propynyloxy)-benzenehexanamide (MSPPOH) |
Cayman chemical Bertin Bioreagent, Dietrich et al., 2009
|
75,770 |
|
| Chemical compound, drug |
Grammostola spatulata mechanotoxin 4 (GsMTx4) |
Alomone Labs, John et al., 2018
|
STG-100 |
|
| Chemical compound, drug |
YODA1 |
Bertin Bioreagent, Lhomme et al., 2019
|
SML1558 |
|
| Chemical compound, drug |
ATPγS |
Tocris Biotechne, Kukulski et al., 2009
|
4080 |
|
| Chemical compound, drug |
4-hydroxy-2,2,6,6-tetramethylpiperidine (TEMPOL) |
Sigma Aldrich Merck, Freidja et al., 2014
|
176,141 |
|
| commercial assay or kit |
Nitric oxide metabolite detection kit |
Cayman Chemical |
780,051 |
|
| commercial assay or kit |
Hydrogen peroxide assay kit |
Abcam |
Ab102500 |
|
| commercial assay or kit |
ATP determination kit |
Invitrogen Molecular Probes |
A22066 |
|
