Fluorescent spectra of His‐tagged GFP showing the change of emission intensity of the solution purified with
(A)
Non‐magnetic Ni‐NTA,
(B)
Magnetic Ni‐NTA,
(C)
Fe3 O4 /MPS@PAA/NTA‐Ni2+ nanocomposites. (a) Before and (b) After reaction with beads, (c) Released His‐tagged GFP in imidazole solution