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Reuse of Fe3 O4 /MPS@PAA/NTA‐Ni2+ in His‐tagged GFP separation
(a) SDS‐PAGE analysis of cell lysate containing His‐tagged GFP (line 1) and proteins released from Fe3 O4 /MPS@PAA/NTA‐Ni2+ nanocomposites reused six times (lanes 3–8). Lane M, the protein molecular weight marker; Lane 2, the supernatant after treatment with Fe3 O4 /MPS@PAA/NTA‐Ni2+, (b) Purification and recycling of Fe3 O4 /MPS@PAA/NTA‐Ni2+ nanocomposites
