FIG. 1.

Immunoblotting of B. bigemina merozoite lysate with antibodies to the p200 antigen. B. bigemina merozoite proteins were separated by SDS-PAGE through a 7.5 to 17.5% gradient gel, transferred to a nitrocellulose membrane, and probed with B. bigemina hyperimmune serum BJ28 diluted to 1:100 (lane 1), 20 μg of bovine recovery serum immunoglobulins per ml (lane 2), polyclonal rat antisera to p200 diluted to 1:50 (lane 3), and 10 μg each of anti-p200 MAbs Bb F4/86.6 (lane 4), Bb F4/86.34 (lane 5), and Bb F4/86.11 (lane 6) per ml. The positions of molecular mass standards are indicated to the left.