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. 2000 Jun;38(6):2240–2247. doi: 10.1128/jcm.38.6.2240-2247.2000

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Copyright © 2000, American Society for Microbiology

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FIG. 5 — SDS-PAGE and immunoblot analyses of p200 recombinant antigen. (A) One microgram of Bb3.5-GST fusion protein preparation was analyzed by SDS-PAGE and stained with Coomassie blue (lane 1). The bracket indicates a small amount of a high-molecular-weight protein. Five micrograms of GST was also electrophoresed (lane 2). (B) Immunoblot of gel arranged as in panel A and probed with MAb Bb F4/86.6. (C) Two micrograms of GST (lane 1) and 10 μg each of C1A-GST (lane 2) and C3A-GST (lane 3) were analyzed by SDS-PAGE and stained with Coomassie blue. (D) Immunoblot of gel arranged as for panel C and probed with hyperimmune serum BJ28. (E) Immunoblot of gel arranged as for panel C and probed with MAb Bb F4/86.6. The sizes of molecular mass standards (lanes M) are shown.