Figure 1.

Workflow of the post-mortem MRI-pathology pipeline. Once the donors were included in the study, they received a post-mortem in situ 3T MRI, and cortical thickness was calculated with FreeSurfer²⁵ from the 3D T1w image (purple box). After the MRI scan, autopsy was performed, and brain tissue was processed for immunohistochemistry against Aβ, p-tau and CD68 (yellow boxes), which were quantified using ImageJ. The correlation between cortical thickness and %area of immunoreactivity was investigated via linear mixed models (dashed grey arrow). Aβ, amyloid-beta; IHC, immunohistochemistry; p-tau, phosphorylated-tau.