Alomari 2015.

Study characteristics
Patient Sampling	Method of sampling: selected Included conditions: no cavitation and enamel lesions Teeth: permanent premolars and molars Sealants: unclear Surface: occlusal
Patient characteristics and setting	Age: not reported Sex: not reported Ethnicity: not reported Country: Kuwait Setting: extracted teeth Number of participants/teeth/sites: 160 teeth Prevalence: enamel 0.89, dentine 0.38
Index tests	Category of test: combined visual, radiograph, and DIAGNOdent Sequence of test(s): examination 1: visual only, examination 2: visual with radiographs, examination 3: visual, radiographs, and DIAGNOdent Examiner training and calibration: yes Teeth cleaning prior to examination: prophylaxis brush using pumice slurry Tooth drying prior to examination: dried for 5 seconds Threshold applied: manufacturer's instructions Device specifics: tip not reported
Target condition and reference standard(s)	Category: histology Sequence of index test and reference standard: following index test Training of examiner: calibration performed Blinding to index test: unclear Multiple tests: no Site selection: highest score from sectioned tooth Target condition: (Downer): sound, outer half of the enamel, inner half of the enamel, outer half of the dentine, inner half of the dentine
Flow and timing	Participants with index test but no reference standard: 0 Participants with reference standard but no index test: 0 Time interval between tests: 1 month Participants receiving both tests but excluded from results: 0
Comparative
Notes	Data for the enamel caries threshold provided by author
Methodological quality
Item	Authors' judgement	Risk of bias	Applicability concerns
DOMAIN 1: Patient Selection
Was a consecutive or random sample of patients enrolled?	No
Was a case‐control design avoided?	Yes
Did the study avoid inappropriate exclusions?	Yes
Could the selection of patients have introduced bias?		High risk
Are there concerns that the included patients and setting do not match the review question?			High
DOMAIN 2: Index Test (All)
Were the index test results interpreted without knowledge of the results of the reference standard?	Yes
If a threshold was used, was it pre‐specified?	Yes
If multiple tests were applied were different examiners used for each (in vivo)?
Could the conduct or interpretation of the index test have introduced bias?		Low risk
Are there concerns that the index test, its conduct, or interpretation differ from the review question?			High
DOMAIN 2: Index Test (Green fluorescence)
DOMAIN 2: Index Test (Blue fluorescence)
DOMAIN 2: Index Test (Red fluorescence)
Were the index test results interpreted without knowledge of the results of the reference standard?	Yes
If a threshold was used, was it pre‐specified?	Yes
If multiple tests were applied were different examiners used for each (in vivo)?
Could the conduct or interpretation of the index test have introduced bias?		Low risk
Are there concerns that the index test, its conduct, or interpretation differ from the review question?			High
DOMAIN 3: Reference Standard
Is the reference standards likely to correctly classify the target condition?	Yes
Were the reference standard results interpreted without knowledge of the results of the index tests?	Yes
Could the reference standard, its conduct, or its interpretation have introduced bias?		Low risk
Are there concerns that the target condition as defined by the reference standard does not match the question?			Low concern
DOMAIN 4: Flow and Timing
Was there an appropriate interval between index test and reference standard?	Yes
Did all patients receive the same reference standard?	Yes
Were all patients included in the analysis?	Yes
Could the patient flow have introduced bias?		Low risk