Kouchaji 2012.

Study characteristics
Patient Sampling	Method of sampling: unclear Included conditions: no cavitation or early lesions, "The study used first permanent molars with and without carious lesions," unclear what level of caries they aimed to recruit Teeth: permanent first molars Sealants: not reported Surface: occlusal
Patient characteristics and setting	Age: 7 to 12 years, mean 9.5 Sex: 21 male, 19 female Ethnicity: not reported Country: Syria Setting: dental hospital Number of participants/teeth/sites: 40 participants/156 teeth Prevalence: enamel 0.85, dentine 0.29
Index tests	Category of test: DIAGNOdent Sequence of test(s): visual (reference standard) then DIAGNOdent Examiner training and calibration: not reported Teeth cleaning prior to examination: "No prior professional cleaning" Tooth drying prior to examination: 3 to 5 seconds Threshold applied: 0‐14 sound, 15‐20 enamel, 21+ dentine Device specifics: tip A
Target condition and reference standard(s)	Category: visual Sequence of index test and reference standard: reference standard then index tests Training of examiner: not reported Blinding to index test: not reported Multiple tests: no Site selection: occlusal surface Target condition: Ekstrand criteria
Flow and timing	Participants with index test but no reference standard: 0 Participants with reference standard but no index test: 0 Time interval between tests: minimal Participants receiving both tests but excluded from results: 0
Comparative
Notes
Methodological quality
Item	Authors' judgement	Risk of bias	Applicability concerns
DOMAIN 1: Patient Selection
Was a consecutive or random sample of patients enrolled?	Unclear
Was a case‐control design avoided?	Yes
Did the study avoid inappropriate exclusions?	Unclear
Could the selection of patients have introduced bias?		Unclear risk
Are there concerns that the included patients and setting do not match the review question?			Low concern
DOMAIN 2: Index Test (All)
Were the index test results interpreted without knowledge of the results of the reference standard?	No
If a threshold was used, was it pre‐specified?	Yes
If multiple tests were applied were different examiners used for each (in vivo)?
Could the conduct or interpretation of the index test have introduced bias?		Low risk
Are there concerns that the index test, its conduct, or interpretation differ from the review question?			Low concern
DOMAIN 2: Index Test (Green fluorescence)
DOMAIN 2: Index Test (Blue fluorescence)
DOMAIN 2: Index Test (Red fluorescence)
Were the index test results interpreted without knowledge of the results of the reference standard?	No
If a threshold was used, was it pre‐specified?	Yes
If multiple tests were applied were different examiners used for each (in vivo)?
Could the conduct or interpretation of the index test have introduced bias?		Low risk
Are there concerns that the index test, its conduct, or interpretation differ from the review question?			Low concern
DOMAIN 3: Reference Standard
Is the reference standards likely to correctly classify the target condition?	No
Were the reference standard results interpreted without knowledge of the results of the index tests?	Yes
Could the reference standard, its conduct, or its interpretation have introduced bias?		High risk
Are there concerns that the target condition as defined by the reference standard does not match the question?			Low concern
DOMAIN 4: Flow and Timing
Was there an appropriate interval between index test and reference standard?	Yes
Did all patients receive the same reference standard?	Yes
Were all patients included in the analysis?	Yes
Could the patient flow have introduced bias?		Low risk