Mepparambath 2014.

Study characteristics
Patient Sampling	Method of sampling: selected Included conditions: non‐cavitated and enamel lesions, "primary molars without obvious cavities were identified in children" Teeth: primary molars Sealants: unclear Surface: approximal
Patient characteristics and setting	Age: 3 to 10 years Sex: not reported Ethnicity: not reported Country: India Setting: dental school Number of participants/teeth/sites: 101 teeth/169 sites Prevalence: enamel 0.22, dentine 0.08
Index tests	Category of test: DIAGNOdent Sequence of test(s): index test then reference standard Examiner training and calibration: not reported Teeth cleaning prior to examination: rubber cup with pumice Tooth drying prior to examination: 3‐way syringe Threshold applied: calculated within study: 0‐9 sound, 10‐17 enamel, 18+ dentine Device specifics: probe A
Target condition and reference standard(s)	Category: bitewing radiograph Sequence of index test and reference standard: index test then reference standard Training of examiner: not reported Blinding to index test: not reported Multiple tests: no Site selection: marked on tooth then sectioned Target condition: sound, outer enamel, inner enamel, past EDJ
Flow and timing	Participants with index test but no reference standard: 0 Participants with reference standard but no index test: 0 Time interval between tests: minimal Participants receiving both tests but excluded from results: 0
Comparative
Notes
Methodological quality
Item	Authors' judgement	Risk of bias	Applicability concerns
DOMAIN 1: Patient Selection
Was a consecutive or random sample of patients enrolled?	No
Was a case‐control design avoided?	Yes
Did the study avoid inappropriate exclusions?	Yes
Could the selection of patients have introduced bias?		High risk
Are there concerns that the included patients and setting do not match the review question?			Low concern
DOMAIN 2: Index Test (All)
Were the index test results interpreted without knowledge of the results of the reference standard?	Yes
If a threshold was used, was it pre‐specified?	Yes
If multiple tests were applied were different examiners used for each (in vivo)?
Could the conduct or interpretation of the index test have introduced bias?		Low risk
Are there concerns that the index test, its conduct, or interpretation differ from the review question?			Low concern
DOMAIN 2: Index Test (Green fluorescence)
DOMAIN 2: Index Test (Blue fluorescence)
DOMAIN 2: Index Test (Red fluorescence)
Were the index test results interpreted without knowledge of the results of the reference standard?	Yes
If a threshold was used, was it pre‐specified?	Yes
If multiple tests were applied were different examiners used for each (in vivo)?
Could the conduct or interpretation of the index test have introduced bias?		Low risk
Are there concerns that the index test, its conduct, or interpretation differ from the review question?			Low concern
DOMAIN 3: Reference Standard
Is the reference standards likely to correctly classify the target condition?	No
Were the reference standard results interpreted without knowledge of the results of the index tests?	Unclear
Could the reference standard, its conduct, or its interpretation have introduced bias?		High risk
Are there concerns that the target condition as defined by the reference standard does not match the question?			Low concern
DOMAIN 4: Flow and Timing
Was there an appropriate interval between index test and reference standard?	Yes
Did all patients receive the same reference standard?	Yes
Were all patients included in the analysis?	Yes
Could the patient flow have introduced bias?		Low risk