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CSC characterization of the KYSE-30 cell spheres. The sphere enrichment process of CSCs is shown (a), the sphere was developed from passage 1 to 3. (b) Upregulation of the pluripotency regulators (SOX2 and OCT4) in spheres of KYSE-30 cells compared to the attached cells. ∗∗p < 0.01. Gene expression was normalized to the GAPDH gene expression as a reference gene, followed by the 2−Δct formula.
