Fig. 1.

Construction and analysis of SARS-CoV-2 spike expression in AdCoV2 vaccines AdCoV2-S and AdCoV2-SdTM were constructed as described in the Materials and Methods, and their brief genetic maps are shown (A). Uninfected 293 cells (mock) and cells infected individually with Ad-LacZ, AdCoV2-S, and AdCoV2-SdTM at an MOI of 0.1 for 24 h were used. Parts of the culture medium (D) and the lysate were harvested. Lysates were extracted to obtain cytosolic (B) and membrane (C) fractions as described in the Materials and Methods. The prepared samples (B, C, and D) were mobilized in SDS-PAGE electrophoresis and antigenically transferred onto the western blot membrane, then blotted with S-specific antibodies, (B and C) Mab5 or (D) COVID-19, 2019-nCoV. The target proteins marked by arrow were shown. Specific antibodies against β-actin, ATP1A1, and mouse IgG heavy chain were used as sample controls for the immunoblots of the cytosolic fraction, membrane fraction, and culture medium, respectively. The data are representative of the results derived from two independent experiments.