Fig. 3.
Induction of the anti-SARS-CoV-2 IgA and IgG subclass by AdCoV2 vaccines. Sera and vw from BALB/c immunized twice with 1 × 107 pfu Ad-LacZ, AdCoV2-S, or AdCoV2-SdTM via i.n. or AdCoV2-S via s.c. at 14-day intervals were set to detect the subclass of specific Ig. (A) vw was collected at 14 days or 1 month and (B) BALF was collected at 6 days after the second immunization. 1/10 or 1/40-diluted individual vw and BALF were then subjected to recombinant intact S protein-immobilized ELISA for IgA detection. For SARS-CoV-2-specific (C) IgG1 IgG2a detection, diluted immune sera were examined using recombinant intact S protein-immobilized ELISA. Anti-mouse IgG1 or IgG2a conjugated with HRP was used as the detected antibody. (D) The ratio of IgG1/IgG2a based on the titer obtained from (B) was calculated. The results from 2 independent experiments were combined and presented. Eleven mice per group were included.