Fig. 9.
Induction of pre-existing anti-Ad antibody following immunization of AdCoV2 vaccine in hamster. Hamster were pre-injected i.m. with 1.5 × 108 pfu of AdLacZ (PEI) at day 0 and then primed/boosted (3 × 107 pfu per dose, 2-week interval) with AdCoV2-S i.n. or s.c. at day 21 and day 35 after 3 weeks of PEI injection(A). Another group of hamster immunized with AdCoV2-S i.n. or s.c. without pre-treatment of PEI was included. The serum at day 20 before AdCoV2-S injection and at day 65 (post 1 month of AdCoV2-S immunization) were taken for subsequent (B) anti-Ad and (C) anti-S antibodies detection by ELISA that was described under the methods.