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. 2021 Jul 23;144(11):3477–3491. doi: 10.1093/brain/awab242

Figure 3.

Figure 3

Intra-striatal α-syn-PFF injections lead to time-dependent onset of motor impairment in the grid-walking task and to early onset of anxiety-like behaviours. (A and B) Graphs of the performance of α-syn-PFF-injected rats in the grid-walking task showing increasing latency to climb (A) and immobility time (B) at 6 and 12 weeks after α-syn-PFF injection compared with sham rats; note that comparisons were significant 12 weeks after the injection (latency to climb and immobility time sham n = 14, α-syn-PFF 6 weeks n = 11; α-syn-PFF 12 weeks n = 10, *P < 0.05, mean ± SEM. One-way ANOVA followed by Bonferroni’s post hoc test). (C) Representative track plots of the route to the top of the grid for sham rats (left) and α-syn-PFF-injected rats 6 weeks (centre) and 12 weeks (right) post-injection. (D–F) Graphs of the distance travelled (D), immobility time (E) and time spent in the centre of the arena (F) of α-syn-PFF-injected and sham rats tested using the open field task. Note the reduced time spent in the centre of the arena for α-syn-PFF-injected rats with respect to Shams in the second part of the test (300–600 s), suggesting anxiety-like behaviour (sham n = 15, α-syn-PFF 6 weeks n = 8, α-syn-PFF 12 weeks n = 16) *P < 0.05, mean ± SEM. One-way ANOVA followed by Bonferroni’s post hoc test. (G) Representative track plots of rats performing the open field test. Note the reduced time spent in the centre of the arena of α-syn-PFF-injected rats at 6 and 12 weeks after the injection with respect to shams.