Figure 5. The IGF-Trap inhibits the growth of pancreatic carcinoma liver metastases.

Experimental liver metastases were generated by inoculation of 1x10⁵ (for males, A-D) or 5x10⁵ (for females, E-H) LMP cells via the intrasplenic/portal route. The number of mice injected was 10 (vehicle) and 9 (IGF-Trap) male mice and 9 (vehicle) and 10 (IGF-Trap) female mice. Treatment with 5 mg/kg IGF-Trap was initiated 1 day later and continued twice weekly for a total of 5 injections per mouse (I). Mice were sacrificed 21 days post tumor inoculation and visible metastases on the surface of the liver enumerated prior to fixation. Results are based on 2 experiments each for male and female mice. Shown in (A&E) are the numbers of metastases per each liver. Shown in (B&F) are representative livers from each group where arrows denote visible metastases. Shown in (C&G) are the mean diameters of metastases per liver and in (D&H) representative images of H&E stained FFPE liver sections (n=9). Scale bar corresponds to 100 µm. Box and whiskers graphs: the box extends from the 25^th to 75^th percentiles, the middle line denotes de median and the whiskers extends from the minimum to the maximum value. T-tumor; L-liver; *p < 0.05; **p < 0.01.