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. 2021 Dec 9;7(12):1579–1588. doi: 10.1038/s41477-021-01037-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — a, MTL/ZmPLA1/NLD structure with the CRISPR-Cas9 target sites shown. mtl/zmpla1/nld had both a 2-bp deletion and a 27-bp insertion in its target region, causing 89 changed amino acids starting from the mutation site and resulting in premature translation termination. b, ZmDMP structure with the CRISPR-Cas9 target sites shown. zmdmp had a 1-bp deletion in its target region, causing 41 changed amino acids starting from the mutation site and resulting in premature translation termination. Mutation sites of the knockout lines are shown in the alignment comparison with the wild-type (WT) sequence, respectively. The target sequences are underlined, with the protospacer-adjacent motif shown in bold type. Insertions are shown in dark red and deletions are shown by dark red dashes.