Fig. 3. Composition of the stromal microenvironment of lung adenocarcinomas.

A UMAPs based on the top 20 principal components of all stromal single-cell transcriptomes split by tissue type, color-coded by cell cluster; and relative quantification of endothelial and fibroblastic/muscle cell clusters per tissue type and, for tumor samples, per patient. B Average gene expression of selected marker genes for stromal cell clusters, for cell cluster color code see (A). C Differentially expressed genes of fibroblastic/muscle cell clusters, maximum top ten genes showed per cell cluster, for cell cluster color code see (A), black arrowheads indicate relevant marker genes of myofibroblast cluster 2 mentioned in the main text. D Mean pathway activity scores of different fibroblastic/muscle cell clusters, mesothelial cells excluded, black arrowheads indicate relevant pathways of myofibroblast clusters 1 and 2 mentioned in the main text. E Correlation of the relative quantity of myofibroblast clusters 1 and 2, color-coded by patient; Spearman’s correlation statistics, linear regression line. F Immunohistochemical staining of CTHRC1 as marker for myofibroblast cluster 2 (see also (C)), quantification of proportion of stromal areal covered by CTHRC1+ cells, mean ± s.d., n = 10 per patient, for patient color code see (E); Pearson’s correlation statistics and linear regression line using mean values per patient.