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. 2021 Dec 9;186:121–138. doi: 10.3897/phytokeys.186.74086

Table 2.

Properties of the two candidate DNA barcoding loci in Ficuskrishnae and Ficusbenghalensis

Parameter ITS2 trnH-psbA
PCR Success (%) 100 100
Sequencing success (%) 100 100
Conserved Sites (%) 91 84.5
Variable informative sites (%) 7.4 13.5
Parsimony-informative sites (%) 8.4 15.5
Aligned length (bp) 309 382
No: of Indels 1 26
Identical sites (%) 76.6 29.5
Transition and Transversion bias (R) 0.446 0.51
Mean inter-specific distance (%) 5.7 ± 3.2 20.8 ± 12.2
Mean intra-specific distance (%) 0.2 ± 0.1 0.3 ± 0.1
Theta prime 0.161 ± 0.069 0.494 ± 0.321
BIC model HKY TPM3uf+G
Resolution of species (%) 100 100