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. 2021 Dec 16;4:1405. doi: 10.1038/s42003-021-02923-3

Fig. 5. Tissue-specific activation of IQ-Switch can be attained by using a discrete cell-type-specific promoter.

Fig. 5

a Schematic illustration of a driver under the control of elavl3 promoter and respective effectors harboring different numbers of QUAS. The driver constructs were equipped with 8.7 kb of elavl3 promoter instead of ubb promoter. Teb (50 µM) was administered for 48 h before imaging at 5 dpf. b Genetic cross of F1 Tg(elavl3:QFDBD-2xAD*-VP16-EcR) with indicated F2 effector lines. The siblings were exposed to Teb to induce ZsGreen transgene expression. Note that the intensity of ZsGreen reporter fluorescence in the pan-neuronal tissues driven by elavl3 promoter was gradually augmented in the order of the increased number of QUAS repeats. c Genetic cross of F1 Tg(elavl3:QFDBD-2xAD*-VP16*) with indicated F2 effector lines. The intensity of ZsGreen fluorescence among the siblings was most prominent with an effector harboring a 13xQUAS transgene. Abbreviations: Teb; tebufenozide. Scale bar: 200 μm.