TABLE 2.
Temperature ramps underlying the programmed freezing curve.
| Stage | Temp. (°C) | Duration (Wendering, #6) | Heating | Seeding | Temperature control |
| 1 | 0.0 | 3.0 | Off | ||
| 2 | 0.0 | 10.0 | Off | ||
| 3 | –10.0 | 14.0 | Off | active | |
| 4 | –50.0 | 0.5 | Off | active | |
| 5 | –20.0 | 5.0 | On | ||
| 6 | –35.0 | 10.0 | Off | ||
| 7 | –100.0 | 13.0 | Off | ||
| 9 | –100.0 | 2.0 | Off |
Within each ramp, the temperature is maintained for specified time before the program adjusts the temperature of the freezing chamber to the next stage. In stage 5, the heat generated by recrystallization during ice formation is counteracted for a short time (30 s) with a jump to lower temperature (−50°C).