Table 2.

Global Panx3 knockout (KO) mouse model generation and general properties

Mouse model	Background	Exon deleted	Model generation	Viability and fertility
Moon, Penuela [16]	C57BL/6 N	2	Embryonic stem cells containing a Panx3 targeting vector (“Knockout-First” reporter tagged insertion promoter-driven cassette; KnockOut Mouse Project Repository) were used to create Panx3-floxed allele (Panx3fl/fl) mice. Panx3fl/fl mice were crossed with C57BL/6 J CMV-Cre-deleter mice (Cre under the control of the cytomegalovirus minimal promoter). Cre recombinase and any C57BL/6 J markers were out-bred after multiple crosses to ensure the mice were congenic to C57BL/6 N WT controls	Reduced litter sizes compared to WT. Heterozygous (Panx3±) crosses produced fewer KO pups than predicted by Mendelian ratios (8% instead of 25%). No difference in weight or size, body composition in aged mice, or increased mortality with ageing compared to WT
Oh [17]	C57BL/6 N	2	The same Panx3 targeting vector used by Moon, Penuela et al. was used to create Panx3fl/fl mice. Panx3fl/fl mice were then crossed with EIIa-Cre transgenic mice, where Cre recombinase is under the control of the adenovirus EIIa promoter	Normal
Ishikawa [26]	C57BL/6	1	Panx3 deletion resulted from the insertion of a PMK3 Panx3-targeting vector (Invitrogen) into W4 embryonic stem cells. The targeting vector consisted of a 10-kb EcoR1 genomic fragment containing Panx3 exon 1 from bac clone RPCI22-47H21. Once heterozygotes (Panx3±) were developed, they were backcrossed for five generations to C57BL/6 mice before full KO mice were bred	None mentioned other than smaller body size
Yorgan [27]	C57BL/6 J	2	Panx3fl/fl mice (Panx3tm1a(KOMP)Wtsi, created using the same targeting vector as Moon, Penuela et al.) were obtained from the Mouse Biology Program (University of California, Davis) and mated with CMV-Cre transgenic mice (B6·C-Tg(CMV-cre)1Cgn/J) to achieve ubiquitous Panx3 deletion	Panx3 KO embryos followed Mendelian ratios just before birth at embryonic day 19.5, but 30% of Panx3 KO pups died shortly after birth