FIG. 1.

Detection of purified Cyclospora cayetanensis oocysts by PCR using FTA filter disks as a template matrix. Serial dilutions of pure C. cayetanensis oocysts were prepared and applied to FTA filter disks in a 10-μl volume. Filters were then processed and used as template in a nested PCR analysis as described in Materials and Methods. (A) Analysis of the first-round PCR products using the primer pair F1E and R2B. The PCR product size is 636 bp. (B) Analysis of the second-round PCR products; 5 μl of first-round products was used as the template for PCR amplification with primer pair F3E and R4B in a 100-μl reaction volume as described in Materials and Methods. PCR amplification with primers F3E and R4B resulted in a 294-bp product.