FIG. 2.

Utility of FTA filters as a template matrix for the PCR detection of other parasitic protozoa. Serial dilutions were prepared from isolates of C. parvum oocysts (A) and E. intestinalis spores (B) and spotted onto FTA filter disks. FTA filters were then processed and used as the template in PCRs as described in Materials and Methods. The primer pair CPB-DIAGF and CPB-DIAGR was used for the detection of C. parvum oocysts and resulted in a 435-bp product. The primer pair SINTF1 and SINTR was used for the detection of E. intestinalis spores and resulted in a 520-bp product.