Fig. 8. MC stabilizers reduce SARS-CoV-2-induced inflammation.
C57BL/6N-Ace2em2(hACE2-WPRE,pgk-puro)/CCLA mice were prior- administered with or without Eba. (5 mg/kg) or Lor. (10 mg/kg) via i.p. 1 day before intranasal infection with SARS-CoV-2 (strain 107) at a dose of 2 × 106 TCID50, and the Eba. and Lor. treatments were continued each day over the couse of infection. 5 mice for each treatment groups, and 2 mice without infection and drug treatment were used as the mock controls. Mice were euthanized and lung lobes were harvested for analysis. a The mRNA levels of IL-6, TNF-α, CCL20, CCL5, IL-8, IL-1β, IFN-γ and CRP were quantified with q(RT-) PCR, and normalized to gapdh mRNA. b Viral replication was monitored by quantifying the expression of nucleocapsid gene. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 are considered significant differences