Figure 5.

Effects of chidamide in genome-wide gene expression and relative pathway in DOHH2 cells. RNA-seq was performed to profile genome-wide gene expression in DOHH2 cells treated with 5μM chidamide for 24 h. (A) Volcano plot depicting 4114 and 2095 genes significantly upregulated and downregulated compared with the DMSO control groups, respectively. (B) Gene Ontology (GO) enrichment analysis of DEGs was performed by Gene Set Enrichment Analysis (GSEA). Pathways affected by chidamide in the DOHH2 cell line. (C) Differential signaling pathways in KEGG pathway enrichment analysis showing genes involved in PI3K/AKT signaling were significantly enriched. (D) Heat map of differentially expressed genes in DOHH2 cells in response to chidamide for 24 h.