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. 2021 Dec 3;12:768693. doi: 10.3389/fpls.2021.768693

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Copyright © 2021 Yu, Gonzalez, Dong, Shan, Tan, Koh, Zhang, Zhu, Dufresne, Martin and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transient expression assays of NAC and SE genes in N. benthamiana leaves in response to different PTI-inducing treatments at different time-points. (A) Schematic workflow of the assay and interpretation of the “overlapping circle” pattern permitted for a qualitative analysis of the GUS histochemical stain. Each leaf was infiltrated with four agrobacterium constructs: YFP (negative control), 35S:GUS (positive control), NACp:GUS and SEp:GUS; (B) N. benthamiana leaves treated with negative control buffer, flg22, and P. fluorescens for 12 h and then GUS staining for 16 h; and (C) N. benthamiana leaves treated with flg22 for 6, 12, and 24 h and then GUS staining for 16 h.