LAM detection assay results according to the ZN score. TB, tuberculosis. ZN−, no AFB per 300 fields; ZN+/−, 1 to 9 AFB per 100 fields; ZN+, 10 to 99 AFB per 100 fields; ZN 2+, 1 to 10 AFB per field in at least 50 fields; ZN 3+, more than 10 AFB per field in at least 20 fields. •••••, cutoff value for positivity (A450 = 0.150). The cutoff value is slightly higher than the A450 + 2 SDs of the sputum samples from 16 nontuberculous Dutch patients (0.126). n, number of sputum samples in each group.