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. 2021 Dec 3;8:769535. doi: 10.3389/fmed.2021.769535

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Copyright © 2021 An, Zhao, Wang, Chu, Xu, Ding, Han and Shang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The phylogenetic reconstruction of HIV-1 CRF01_AE in Shenyang during 2002–2017. The maximum likelihood tree was constructed using 2014 HIV-1 CRF01_AE pol sequences (HXB2:2253-3314 nt) sampled in Shenyang under GTR+I+G4 model with 1000 replicates in IQ-tree. The tree is rooted with three subtype A sequences as outgroup and the reference sequences (ref.5) were used to define CRF01_AE. Six lineages were identified, of which four were reported previously. Four colored circles represent risk factor, sex, household register and lineage from inner to outer.