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. 2021 Dec 3;12:765741. doi: 10.3389/fimmu.2021.765741

Figure 4.

Figure 4

Low-salt diet reduces severity in K/BxN serum transfer-induced arthritis and is as effective as IL-1 receptor blockade. Mice were exposed to low salt (LS), regular salt (RS), and high salt (HS) diets for 2 weeks before serum transfer and continued for further 8 days. (A) Mean arthritis score in STIA mice on different diets (LS, n = 10; HS, n = 10; RS, n = 10). *p < 0.05, **p < 0.01,****p < 0.0001; blue asterisk: LS versus RS, black asterisk: LS versus HS. p-values were calculated by two-way ANOVA followed by Bonferroni’s multiple comparisons test. (B) Area under the curve of mean arthritis score. **p < 0.01; ns = non-significant. p-values were calculated by one-way ANOVA followed by Bonferroni’s multiple comparisons test. (C) LS diet is as effective as treatment with IL-1 receptor antagonist protein (anakinra) in HS diet mice. One group of mice under HS diet received i.p. injections of 100 mg/kg anakinra simultaneously with arthritis induction as well as on days 1, 2, and 3 (LS, n = 4; HS, n = 4; HS +Anakinra, n = 4). Red asterisk: LS versus HS, gray asterisk: HS/anakinra versus HS. ***p < 0.001, ****p < 0.0001. p-values were calculated by two-way ANOVA followed by Bonferroni’s correction. (D) Area under the curve of mean arthritis score of STIA mice undergoing LS diet, HS diet, and HS plus anakinra.