Table 1.
Compositions of cell culture medium containing different concentrations of NaCl and mannitol.
| Composition of solutions | |||
|---|---|---|---|
| Low salt (LS) medium | NaCl (mM) added | mOsm/kg | Name |
| LS | 0 | 98 | LS0 |
| LS | 25 | 150 | LS25 |
| LS | 50 | 202 | LS50 |
| LS | 100 | 299 | LS100 |
| LS | 140 | 387 | LS140 |
| Low salt (LS) medium | Mannitol (mM) added | mOsm/kg | Name |
| LS | 20 | 112 | LSM20 |
| LS | 70 | 165 | LSM70 |
| LS | 100 | 198 | LSM100 |
| LS | 200 | 289 | LSM200 |
| LS | 280 | 361 | LSM280 |
Low salt (LS) medium was prepared as described elsewhere (24), supplemented with respective concentrations of NaCl and used for in vitro assays as follows: LS0 contains 25 mM NaCl (LS25 ≙ 50 mM NaCl, LS50 ≙ 75 mM NaCl, LS100 ≙ 125 mM NaCl, LS140 ≙ 165 mM NaCl). LS100 corresponds to regular salt conditions, LS140 to high salt conditions, and ≤ LS50 to low salt conditions. To compare effects of NaCl solutions to an isosmotic substance, LS medium was supplemented with mannitol to obtain equi-osmolalic solutions. Osmolality (mOsm/kg) of respective solutions was measured (third column).