Table 2.
Fold change in mRNA expression in spleens.
| Mouse model | GENE | Diet | p-value | |
|---|---|---|---|---|
| HS | LS | |||
| IL-1beta | 1.018 ± 0.072 | 0.667 ± 0.091 | 0.0079 | |
| IL-17 | 1.266 ± 0.314 | 1.709 ± 0.547 | 0.4930 | |
| MAPK14 | 1.014 ± 0.061 | 0.7163 ± 0.158 | 0.0040 | |
| STIA | NFAT5 | 0.981 ± 0.058 | 0.578 ± 0.120 | 0.0004 |
| SGK1 | 1.023 ± 0.073 | 0.916 ± 0.162 | 0.5580 | |
| NF-kappaB-I-alpha | 1.186 ± 0.2103 | 0.9219 ± 0.178 | 0.3530 | |
| IL-1beta | 1.056 ± 0.118 | 1.324 ± 0.115 | 0.1240 | |
| IL-17 | 1.242 ± 0.812 | 2.092 ± 1.162 | 0.0855 | |
| IFN-gammaday47 | 1.049 ± 0.110 | 1.1 ± 0.119 | 0.8531 | |
| IFN-gammad20 | 1.051 ± 0.171 | 0.689 ± 0.090 | 0.0953 | |
| CIA | IL-23 | 1.124 ± 0.1886 | 0.597 ± 0.093 | 0.0194 |
| MAPK14 | 1.022 ± 0.139 | 0.760 ± 0.077 | 0.1201 | |
| NFAT5 | 1.010 ± 0.085 | 0.831 ± 0.125 | 0.5427 | |
| SGK1 | 1.018 ± 0.209 | 0.908 ± 0.428 | 0.7495 | |
| NF-kappaB-I-alpha | 1.135 ± 0.0552 | 0.824 ± 0.037 | 0.6355 | |
STIA and CIA mice fed with high salt (HS) or low salt (LS) diets. Splenocytes were prepared 8 days post serum transfer and 47 days post CII immunization. Fold change expression was calculated by 2−ΔΔCT. Gene expression was normalized against HPRT. HS group was used as calibrator (STIA: LS, n = 10; HS, n = 10; CIA: LS, n = 10; HS, n = 9). Statistical significance was defined as p ≤ 0.05. p-values were calculated by Student’s t-test. All data were expressed as ± SEM.