FIG. 3.

Pattern of in vivo splicing of 3′ splice site dinucleotide mutants. The indicated minigene constructs were transfected into CHO cells, and total RNA was prepared after 48 h. The splicing pattern of the P120 intron F was determined by RT-PCR amplification and seperation of the products on a denaturing gel. The positions of spliced and unspliced PCR products are shown, as well as the position of a U2-dependent cryptic splice that is activated in some constructs. The numbers at the left indicate the distance in nucleotides between the branch site and the 3′ splice site in each product.