Skip to main content
. Author manuscript; available in PMC: 2022 Jan 5.
Published in final edited form as: Nat Biotechnol. 2021 Jul 5;39(12):1574–1580. doi: 10.1038/s41587-021-00962-z

Figure 4 |. s3 whole genome sequencing (s3-WGS) and genome conformation capture (s3-GCC).

Figure 4 |

(a) Schematic of sci-WGS and sci-GCC library construction. (b) Experimental flow through and plate layout for control GM12878 diploid line (left) and PDAC cell lines (right). (c) Heatmap summary of chromosome count per cell in PDAC-1 SKY data. Example karyotype of PDAC-1 cell (bottom). (d) Boxplot of unique read count per cell for matched GM12878 cell line (n=3,576, 45 cells for sci-WGS and s3-WGS, respectively)10. (e) Boxplot of MAD score per cell per sample and assay (n=111, 698, 257, 57, and 145 cells, listed left to right). (f) Boxplot of reads passing filter per cell. Cell count same as panel e. (g) Comparison boxplot of s3-GCC and sci-HiC distal contacts (≥50kbp) per cell (n=2,312, 202 cells for sci-HiC and s3-GCC, respectively)31. Boxplots depicts median and center quartiles with 10th and 90th percentile whiskers.