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. Author manuscript; available in PMC: 2022 Apr 25.
Published in final edited form as: Nat Nanotechnol. 2021 Oct 25;16(12):1424–1434. doi: 10.1038/s41565-021-00982-5

Extended Data Fig. 2. Early T cell activation reported by cell surface expression of CD69.

Extended Data Fig. 2

a, Quantification of the CD69 expression level in Jurkat T cells expressing WT CAR, defective LiCAR (C+D5 lacking the CD3 subunit), or LiCAR (C+D4) before (open box) and after light illumination (blue box; 20 min and then 10 h with the pulse of 10 s ON + 60 s OFF). Cells were co-incubated with hCD19- negative K562 cells (open box) or hCD19-positive Raji cells (red box).

b, Quantification of CD69 expression in Jurkat T cells co-cultured with hCD19-negative K562 (open box) or hCD19-positive Raji cells (red box). n = 2 independent biological replicates (mean ± range).