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. 2021 Dec 3;12:778098. doi: 10.3389/fimmu.2021.778098

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Copyright © 2021 Hong, Zhu, Yuan, Niu and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The Nitrite concentration decreased and the bacterial load increased in the gastrointestinal tract of shrimp after knockdown of Nos. (A) Nos-RNAi efficiency in the stomach analyzed using qPCR; dsGfp was used as the control. (B) The concentration of nitrite in the stomach of Nos-knockdown shrimp. (C) The bacterial load in the stomach of Nos-knockdown shrimp. (D) Nos RNAi efficiency in the intestines analyzed using qPCR; dsGfp was injected as the control. (E) The concentration of nitrite in intestines of Nos-knockdown shrimp. (F) The bacterial load in the intestines of Nos-knockdown shrimp. Significance differences were analyzed using Student’s t-test and significance was accepted at p < 0.05.