Fig. 2. GPR120-deficient CD4⁺CD45RB^hiT cells induce more severe colitis in Rag^−/− mice.

WT and GPR120-deficient CD4⁺CD45Rb^hiT cells (1×10⁵ cells/mouse) were intravenously transferred to Rag^−/− mice (N=12/group). (A) Mouse weight changes. (B) Representative gross morphology of the colon. (C) Colon length. (D) Representative intestinal H&E staining. (E) Pathological score. (F) Colonic secretion of cytokines. (G) Representative flow cytometry profile of LP CD4⁺T cells. (H–K) Bar charts of IFNγ⁺, IL-17A⁺, IL-10⁺, and Foxp3⁺CD4⁺T cells. All data are pooled from three independent experiments. (D) Scale bar, 300 μm. (A, C, F, and H–K) unpaired Student t-test; (E) Mann–Whitney U test.