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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Genes Cells. 2021 Oct 28;26(12):1014–1022. doi: 10.1111/gtc.12902

Figure 1. Intracellular localization of PTEN-GFP mutants in HCT116 cells.

Figure 1.

(A) HCT116 cells were transfected with the indicated PTEN-GFP proteins and observed by live-cell imaging. (B) The signal intensity of PTEN in the nucleus was quantified relative to the cytosol in HCT116 cells. Values represent the mean ± SD (n =24–51). (C) Phosphatase activity of PTEN mutants purified from HEK293T cells was measured by the amount of phosphate released from PIP3 diC8 per minute using the malachite green assay. The bar represents the mean ± SD (n = 6). Statistical analyses were performed using ANOVA with post hoc Tukey test in (B and C).