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. Author manuscript; available in PMC: 2022 Dec 13.
Published in final edited form as: Cancer Cell. 2021 Oct 21;39(12):1610–1622.e9. doi: 10.1016/j.ccell.2021.09.011

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
αGr-1 BioXcell Cat#BE0075
αLy-6G BioXcell Cat#BE0075-1
αIFNAR-1 BioXcell Cat#BE0241
αIL-4 BioXcell Cat#BE0045
αCD8 BioXcell Cat#BE0061
αNK1.1 BioXcell Cat#BE0036
αIL-21R BioXcell Cat#BE0258
αIFNγ BioXcell Cat#BE0055
αIL-2 BioXcell Cat#BE0043
TRP-1 Santa Crus Cat#sc-166857
β-actin Cell Signaling Cat#3700T
BV421 anti-mouse CD11b BioLegend Cat#101251
PerCP anti-mouse CD11c BioLegend Cat#117326
BV605 anti-mouse F4/80 BioLegend Cat#123133
BV570 anti-mouse Ly6G BioLegend Cat#127629
PE anti-mouse Ly6C BioLegend Cat#128008
FITC anti-mouse CD4 BioLegend Cat#100405
APC anti-mouse CD45.2 BioLegend Cat#109814
PE anti-mouse CD3 BioLegend Cat#100206
PE anti-mouse CD39 BioLegend Cat#143804
PE anti-mouse PD-1 BioLegend Cat#135206
APC anti-mouse Lag3 BioLegend Cat#125210
APC anti-mouse IFNγ BioLegend Cat#505810
APC anti-mouse IL-9 BioLegend Cat#514106
APC anti-mouse IL-17 BioLegend Cat#v506916
PE anti-human IL-9 BioLegend Cat#507603
FITC anti-human CD4 BioLegend Cat#357406
PE anti-human CD39 BioLegend Cat#328208
PE anti-mouse CD44 BioLegend Cat#103024
FITC anti-mouse CD62L BioLegend Cat#104406
PE anti-mouse CD8 BioLegend Cat#100708
APC anti-mouse CD11c BioLegend Cat#117310
Bacterial and virus strains
 
 
Biological samples
Patient-derived xenografts (PDX) Wake Forest Baptist Comprehensive Cancer Center N/A
Chemicals, peptides, and recombinant proteins
The NF-κB-specific inhibitor QNZ Santa Cruz Biotechnology Cat#sc-200675
eATP signaling inhibitor Suramin Santa Cruz Biotechnology Cat#sc-200833
CD39 inhibitor POM-1 Santa Cruz Biotechnology Cat#sc-203205
MHC-I peptide for TRP-2180-188 (SVYDFFVWL) GenScript N/A
MHC-I peptide for gp10025-33 (EGSRNQDWL) GenScript N/A
B16-M27 (MHC-I peptide: REGVELCPGNKYEMRRHGTTHSLVIHD) GenScript N/A
B16-M30 (MHC-II peptide: PSKPSFQEFVDWENVSPELNSTDQPFL) GenScript N/A
B16-M48 (MHC-II peptide: SHCHWNDLAVIPAGVVHNWDFEPRKVS) GenScript N/A
OVA MHC-II peptide (ISQAVHAAHAEINEAGR) GenScript N/A
OVA MHC-I peptide (SIINFEKL) GenScript N/A
MHC class II–restricted TRP1 (SGHNCGTCRPGWRGAACNQKILTVR) GenScript N/A
Mouse IL-1β R&D Systems Cat#NP_032387
Mouse IL-4 R&D Systems Cat#P07750
Mouse IL-6 R&D Systems Cat#P08505
Mouse IL-2 R&D Systems Cat#P04351
Mouse IL-12 R&D Systems Cat#P43432
Mouse IL-23 R&D Systems Cat#P43432
Mouse IL-21 R&D Systems Cat#Q9ES17.1
Human TGF-β1 R&D Systems Cat#P01137
Human IL-4 R&D Systems Cat#204-IL-050/CF
Critical commercial assays
ATP Determination Kit ThermoFisher Cat#A22066
ELISA kits mouse TNF-α eBioscience Cat#50-112-8899
ELISA kits mouse IFN-γ eBioscience Cat#50-112-9023
ELISA kits mouse IL-6 eBioscience Cat#50-112-8808
ELISA kits mouse IL-1β eBioscience Cat#50-112-8706
ELISA kits mouse IFN-β R&D Cat#424001
Annexin V-FITC Apoptosis Detection Kit eBioscience Cat#BMS500FI-100
Deposited data
CD11b cell gene array NCBI Gene Expression Omnibus GSE151712
Experimental models: Cell lines
B16 ATCC Cat#CRL-6323
ID8 A gift from Dr. Neveen Said at Wake Forest School of Medicine N/A
SK-OV-3 ATCC Cat#HTB-77
293T ATCC Cat#CRL-3216
Experimental models: Organisms/strains
C57BL/6 The Jackson Laboratory Cat#000664
CD45.1 (B6.SJL-Ptprca Pepcb/BoyJ) The Jackson Laboratory Cat#002014
Ccr1−/− (B6.129S-Ccr1tm1Gao/AdlJ ) The Jackson Laboratory Cat#032932
Ccr2−/− (B6.129S4-Ccr2tm1Ifc/J ) The Jackson Laboratory Cat#004999
Ccr5−/− (B6;129P2-Ccr5tm1Kuz/J ) The Jackson Laboratory Cat#005427
Ccr6−/− (B6.129P2-Ccr6tm1Dgen/J ) The Jackson Laboratory Cat#005793
Ccr7−/− (B6.129P2(C)-Ccr7tm1Rfor/J ) The Jackson Laboratory Cat#006621
Ccr9−/− (B6N.129-Ccr9tm1Lov/JmfJ) The Jackson Laboratory Cat#027041
Cxcr2−/− (B6.129S2(C)-Cxcr2tm1Mwm/J) The Jackson Laboratory Cat#006848
Cxcr3−/− (B6.129P2-Cxcr3tm1Dgen/J) The Jackson Laboratory Cat#005796
Cxcr5−/− (B6.129S2(Cg)-Cxcr5tm1Lipp/J) The Jackson Laboratory Cat#006659
Cxcr6−/− (B6.129P2-Cxcr6tm1Litt/J) The Jackson Laboratory Cat#005693
Cx3cr1−/− (B6.129P2(Cg)-Cx3cr1tm1Litt/J) The Jackson Laboratory Cat#005582
P2ry2−/− (B6.129P2-P2ry2tm1Bhk/J) The Jackson Laboratory Cat#009132
Ifnar1−/− (B6(Cg)-Ifnar1tm1.2Ees/J) The Jackson Laboratory Cat#028288
Camp−/− (B6.129X1-Camptm1Rlg/J) The Jackson Laboratory Cat#017799
Sting−/− (B6(Cg)-Sting1tm1.2Camb/J) The Jackson Laboratory Cat#025805
Mavs−/− (B6;129-Mavstm1Zjc/J) The Jackson Laboratory Cat#008634
Myd88−/− (B6.129P2(SJL)-Myd88tm1.1Defr/J) The Jackson Laboratory Cat#009088
Tlr4−/− (B6(Cg)-Tlr4tm1.2Karp/J) The Jackson Laboratory Cat#029015
Tlr3−/− (B6;129S1-Tlr3tm1Flv/J) The Jackson Laboratory Cat#005217
Trif−/− (C57BL/6J-Ticam1Lps2/J) The Jackson Laboratory Cat#005037
TRP-1 (B6.Cg-Rag1tm1Mom Tyrp1B-wTg(Tcra,Tcrb)9Rest/J) The Jackson Laboratory Cat#008684
MHCII−/− NSG (NOD.Cg-PrkdcscidH2-Ab1tm1DoiIl2rgtm1Wjl/SzJ) The Jackson Laboratory Cat#021885
Cd4−/− (B6.129S2-Cd4tm1Mak/J) The Jackson Laboratory Cat#002663
MHCI−/− NSG (NOD.Cg-B2mtm1UncPrkdcscidIl2rgtm1Wjl/SzJ) The Jackson Laboratory Cat#010636
Il9r−/− A gift from Dr. Jean-Christophe Renauld at Ludwig Institute for Cancer Research N/A
Oligonucleotides
mIFNa F: 5′-TCAGTCTTCCCAGCACATTG-3′ Sigma N/A
mIFNa R: 5′- GAGAAGAAACACAGCCCCTG-3′ Sigma N/A
mIFNb F: 5′-CAGCTCCAAGAAAGGACGAAC-3′ Sigma N/A
mIFNb R: 5′-GGCAGTGTAACTCTTCTGCAT-3′ Sigma N/A
eMLV spliced F: 5′-CCAGGGACCACCGACCCACCG-3′ Sigma N/A
eMLV spliced R: 5′-TAGTCGGTCCCGGTAGGCCTCG-3′ Sigma N/A
MMTV spliced F: 5′-AGAGCGGAACGGACTCACCA-3′ Sigma N/A
MMTV spliced R: 5′-TCAGTGAAAGGTCGGATGAA-3′ Sigma N/A
xMLV F: 5′-TCTATGGTACCTGGGGCTC-3′ Sigma N/A
xMLV R: 5′-GGCAGAGGTATGGTTGGAGTAG-3′ Sigma N/A
pMLV/mpMLV common F: 5′-CCGCCAGGTCCTCAATATAG-3′ Sigma N/A
pMLV R: 5′-AGAAGGTGGGGCAGTCT-3′ Sigma N/A
mpMLV R: 5′-CGTCCCAGGTTGATAGAGG-3′ Sigma N/A
GLN F: 5′-TGTGTAAGTCCAGACGCAG-3′ Sigma N/A
GLN R: 5′-CCAACCTACTCCAAAAACAG-3′ Sigma N/A
IAP F: 5′-AAGCAGCAATCACCCACTTTGG-3′ Sigma N/A
IAP R: 5′-CAATCATTAGATGCGGCTGCCAAG-3′ Sigma N/A
MMERVK F: 5′-CAAATAGCCCTACCATATGTCAG-3′ Sigma N/A
MMERVK R: 5′-GTATACTTTCTTCTTCAGGTCCAC-3′ Sigma N/A
MaLR F: 5′-ATGTTTTGGGGAGGACTGTG-3′ Sigma N/A
MaLR R: 5′-AGCCCCAGCTAACCAGAAC-3′ Sigma N/A
MusD/EnTII common F: 5′-GTGCTAACCCAACGCTGGTTC-3′ Sigma N/A
MusD R: 5′-CTCTGGCCTGAAACAACTCCTG-3′ Sigma N/A
ETnII R: 5′-ACTGGGGCAATCCGCCTATTC-3′ Sigma N/A
Mervl Pol F: 5′-ATCTCCTGGCACCTGGTATG-3′ Sigma N/A
Mervl Pol R: 5′-AGAAGAAGGCATTTGCCAGA-3′ Sigma N/A
mGAPDH F: 5’-TTGATGGCAACAATCTCCAC-3’ Sigma N/A
mGAPDH R: 5’-CGTCCCGTAGACAAAATGGT-3’ Sigma N/A
hIFNα F: 5′-GCCTCGCCCTTTGCTTTACT-3′ Sigma N/A
hIFNα R: 5′-CTGTGGGTCTCAGGGAGATCA-3′ Sigma N/A
hIFNβ F: 5′-ATGACCAACAAGTGTCTCCTCC-3′ Sigma N/A
hIFNβ R: 5′-GGAATCCAAGCAAGTTGTAGCTC-3′ Sigma N/A
hMSLN F: 5′-CCAACCCACCTAACATTTCCAG-3′ Sigma N/A
hMSLN R: 5′-CAGCAGGTCCAATGGGAGG-3′ Sigma N/A
HERV-F F: 5′-CCTCCAGTCACAACAACTC-3′ Sigma N/A
HERV-F R: 5′-TATTGAAGAAGGCGGCTGG-3′ Sigma N/A
HERV-E F: 5′-GGTGTCACTACTCAATACAC-3′ Sigma N/A
HERV-E R: 5′-GCAGCCTAGGTCTCTGG-3′ Sigma N/A
HERV-W F: 5′-TGAGTCAATTCTCATACCTG-3′ Sigma N/A
HERV-W R: 5′-AGTTAAGAGTTCTTGGGTGG-3′ Sigma N/A
ERV-F2B F: 5′-AAAAAGGAAGAAGTTAACAGC-3′ Sigma N/A
ERV-F2B R: 5′-ATATAAAGACTTAGGTCCTGC-3′ Sigma N/A
HERV-K(HML-2) F: 5′-AAAGAACCAGCCACCAGG-3′ Sigma N/A
HERV-K(HML-2) R: 5′-CAGTCTGAAAACTTTTCTCTC-3′ Sigma N/A
HERV-K(HML-5) F: 5′-TGAAAGGCCAGCTTGCTG-3′ Sigma N/A
HERV-K(HML-5) R: 5′-CAATTAGGAAATTCTTTTCTAC-3′ Sigma N/A
hLINE ORF1 F: 5′-TTGGAAAACACTCTGCAGGATATTAT-3′ Sigma N/A
hLINE ORF1 R: 5′-TTGGCCTGCCTTGCTAGATT-3′ Sigma N/A
hGAPDH F: 5′-AGTCAACGGATTTGGTCGTATTGGG-3′ Sigma N/A
hGAPDH R: 5′-ACGTACTCAGCGCCAGCATCG-3′ Sigma N/A
Recombinant DNA
MART-127-35 specific TCR lentivector (DMF5) a gift from Dr. Steven Rosenberg N/A
PMKO.1 Addgene Cat#10676
MigR1 Addgene Cat#27490
pUltra Addgene Cat#24129
lentiCRISPR v2 Addgene Cat#52961
Software and algorithms
GSEA v2.2.2 Broad Institute http://software.broadinstitute.org/gsea/index.jsp
Other