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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Acta Biomater. 2021 Oct 19;137:53–63. doi: 10.1016/j.actbio.2021.10.021

Figure 4. In vitro cytocompatibility of the hydrogels after photocrosslinking.

Figure 4.

(A) Microscopic images of human corneal epithelial cells (HCECs) incubated for 24 h with culture medium or fluid extracts of photocrosslinked hydrogels prepared with different concentrations of HAGM, GelMA and PEGDA and then stained with calcein-AM (green - viable dye) and propidium iodide (red - dead dye) (n=3 per group), scale bar = 100 μm. (B) Cell viability measured by PrestoBlue assay after 3-days incubation with hydrogels prepared with different concentrations of HAGM, GelMA and PEGDA. Data is represented as mean ± SD (n=3 per group).