Table 1:

Affinity-based interactions and their associated amino acids

Affinity-based interactions	Amino acids with participating side-chains
Non-polar interactions	Ala, Ile, Leu, Met, Phe, Pro, Trp, Val, Tyr
π-π/cation-π interactions	Phe, Trp, Tyr/Lys, His, Arg
H-bonding	Cys, Asp, Glu, His, Lys, Asn, Gln, Arg, Ser, Thr, Trp, Tyr
Electrostatic	Cys, Asp, Glu, His, Lys, Arg, Tyr
Metal ligand	Asp, Arg, Lys, Tyr, Glu, His, Cys