Skip to main content
. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Pain. 2022 Mar 1;163(3):496–507. doi: 10.1097/j.pain.0000000000002382

Fig 3. Effect of ANA12 on OSCC-induced pain behaviors.

Fig 3.

Following baseline (BL) feeding measurements, athymic mice were injected with A. NOK cells or B. HSC2 cells. At day 9 post cell inoculation, NOK and HSC2-injected animals were injected with either vehicle or 3ug/20ul injection of ANA12 in the tongue and 1 hour following injection, food intake was measured for an hour. n=6–10 per group. C. HSC2 cells were grown in serum free conditions and conditioned media (CM) was harvested at 24 hours and filtered. Following baseline (BL) feeding measurements, athymic animals were injected with either vehicle or 3ug/20ul injection ANA12 in the tongue and 1 hour later, 15ul of CM was injected in the tongue of both groups. Feeding behavior was tested 15 mins after CM injection (Post CM Injection). n=6–10 per group D. HSC2 cells were grown in serum free conditions and conditioned media (CM) was harvested at 24 hours and filtered. Baseline (BL) von Frey thresholds were taken for two groups of athymic animals: those that were later assigned to vehicle group [BL(VEH)] and those that were assigned to ANA12 group [BL(ANA12)]. Following BL measurements, animals were injected with either vehicle (VEH) or 3ug/20ul injection of ANA12 in the vibrissal pad and 1 hour later, 15ul of CM was injected in the vibrissal pad in both groups. von Frey thresholds were measured 15 mins after CM injection (VEH, CM and ANA12, CM). n=5 per group. Data are represented as mean±SEM. Data analyzed with one-way ANOVA with Bonferroni post hoc test at p<0.05.