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. 2021 Dec 3;9:767006. doi: 10.3389/fcell.2021.767006

FIGURE 4.

FIGURE 4

CCG targeted TAZ to reduce adipogenic differentiation of BMSCs in vitro. (A) MTT assays presented cell viabilities after CCG administration during adipogenesis of BMSCs. (B, C) Representative images of Oil Red O staining reflected the lipid droplets. Scale bar: 100 μm. (D, E) Relative mRNA levels of TAZ, PPARγ, and perilipin to GAPDH were presented in Day 3 and Day 7 in the absence or presence of CCG during adipogenesis. (F–I) Relative protein levels of TAZ, PPARγ, and perilipin to GAPDH were presented in Day 3, in Day 7, and in Day 14 after the treatment. *p < 0.05 vs. the control group. (J–L) Relative mRNA and protein levels to GAPDH of TAZ were significantly knocked down by the SiTAZ plasmid during adipogenesis. (M–Q) SiTAZ discounted the CCG induced up-expression of TAZ and down-expression of PPARγ and perilipin at Day 3 after the treatment. (R, S) Representative images of Oil Red O staining showed the lipid droplets in different groups. Bar graphs showed the means ± SD from three independent experiments. Scale bar: 100 μm (n = 3) *p < 0.05 vs. the Vehicle group; #p < 0.05 vs. the Vehicle + CCG group.