Table 1.
Antibodies used for flow cytometry, IHC and IF.
| Antibody name | Source | Catalogue number | Dilution |
|---|---|---|---|
| Antibodies used for flow cytometry | |||
| F4/80 | Biolegend | 123116 | (0.5µg)/test |
| CD11b | BD Pharmingen | 560455 | (0.5µg)/test |
| CD86 | Biolegend | 105005 | (0.5µg)/test |
| CD206 (MMR) | eBioscience | 25-2061-82 | (0.5µg)/test |
| IL-4 | BD Pharmingen | 554389 | (0.5µg)/test |
| CD4 | Biolegend | 100516 | (0.5µg)/test |
| Foxp3 | Biolegend | 126419 | (0.5µg)/test |
| Antibodies used for IHC | |||
| Anti-CD68 | Abcam | ab201340 | 1:400 |
| Anti-MCP1 | Abcam | ab7202 | 1:100 |
| HRP conjugated Goat Anti-Mouse IgG (H+L) | Servicebio | GB23301 | 1:200 |
| HRP conjugated Goat Anti-Rabbit IgG (H+L) | Servicebio | GB23303 | 1:200 |
| Antibodies used for IF | |||
| IL-17 antibody | Santa Cruz | 374218 | 1:100 |
| Foxp3 antibody | Santa Cruz | 53876 | 1:100 |
| IFN-γ antibody | R&D | AF-585-NA | 1:100 |
| Anti-Mannose Receptor antibody | Abcam | ab64693 | 1:100 |
| Anti-F4/80 antibody | Abcam | ab16911 | 1:100 |
| Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed | Abcam | ab150081 | 1:1000 |
| Cy3-labeled anti-mouse antibody | Beyotime | A0521 | 1:200 |
| NL557-labeled anti-Goat antibody | R&D | NL001 | 1:200 |