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. 2021 Dec 3;12:733808. doi: 10.3389/fimmu.2021.733808

Table 2.

The primer sequences used in quantitative real-time PCR.

Gene Primer Forward Sequence (5’-3’) Reverse Sequence (5’-3’)
β-actin GCTTCTTTGCAGCTCCTTCG GGCCTCGTCACCCACATAG
iNOS GACATTACGACCCCTCCCAC GCACATGCAAGGAAGGGAAC
TNF-α CACCTGGCCTCTCTACCTTG CGATTACAGTCACGGCTCCC
MCP-1 TTGACCCGTAAATCTGAAGCTAAT TCACAGTCCGAGTCACACTAGTTCAC
Arg-1 ACATTGGCTTGCGAGACGTA ATCACCTTGCCAATCCCCAG
IFN-γ CACGGCACAGTCATTGAAAG CATCCTTTTGCCAGTTCCTC
IL-10 CCAAGCCTTATCGGAAATGA TCCTGAGGGTCTTCAGCTTC
IL-17 GTCCAAACACTGAGGCCAAG ACGTGGAACGGTTGAGGTAG

iNOS, inducible nitric oxide synthase; TNF-α, tumor necrosis factor α; MCP-1, monocyte chemoattractant protein-1; Arg-1, arginase-1; IFN-γ, interferon gamma; IL-10, interleukin 10; IL-17, interleukin 17.