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. 2021 Dec 3;14:742790. doi: 10.3389/fnmol.2021.742790

FIGURE 3.

FIGURE 3

WT tau modulates gene expression of chromatin organization and remodeling factors. (A) Enrichment plot for GO Chromatin organization. (B) Enrichment plot for GO Chromatin remodeling. (C) Enrichment plot for GO-Covalent Chromatin modification. (D) IB of Up-regulated genes: RCC1, DNAJC2 and Histone 1.2 (red box) and Down-regulated genes: SMARCC1, SMARCA5 and HMGB1 (blue box) in cytoplasm and nuclear fractions from WT and P301L tau iHEK. Histone 3 and β-Actin has been used as loading control for nuclear and cytoplasmatic fractions, respectively. (E) Immunoblot quantification of RCC1 (*p < 0.01), DNAJC2 (*p < 0.01), Histone1.2 (*p < 0.01), cytoplasmic HMGB1 (*p < 0.01), nuclear HMGB1 and SMARCA5 proteins in cytoplasmatic and nuclear fractions of WT tau iHEK. (F) Representative Tau 13 (magenta) and Histone 1.2 (green) Co-IF of control (-Tet) and treated WT tau iHEK. (G) Histone 1.2 integrated density quantification in control and + Tet cells (Unpaired t-test, ****p < 0.0001). (H) Representative Tau 13 (magenta) and Ki67 (green) Co-IF of control (-Tet) and treated WT tau iHEK. (I) Ki67 integrated density quantification in control and + Tet cells (Unpaired t-test, ****p < 0.0001). (J) Representative Tau 13 (magenta) and SMARCC1 (green) Co-IF of control (-Tet) and treated WT tau iHEK. (K) SMARCC1 integrated density quantification in control and + Tet cells (Unpaired t-test, ****p < 0.0001). (L) Representative Tau 13 (magenta) and SMARCA5 (green) Co-IF of control (-Tet) and treated WT tau iHEK. (M) SMARCA5 integrated density quantification in control and + Tet cells (Unpaired t-test, ****p < 0.0001).